IL-4 ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT11846
IL-4 ELISA Kit, Human: General Information
Product name
IL-4 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
2.54 pg/mL
Assay range
10.94-700 pg/mL
Specificity
Recognizes both recombinant and natural Human IL-4
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL4 control were assayed for interference. No significant interference was observed.
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 149.00 | 306.00 | 646.00 |
| SD | 5.42 | 13.87 | 19.98 |
| CV(%) | 3.6% | 4.5% | 3.1% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 148.00 | 307.00 | 608.00 |
| SD | 1.00 | 22.03 | 33.87 |
| CV(%) | 0.7% | 7.2% | 5.6% |
Recovery
The recovery of Human IL4 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
| Sample |
| supernatant (n=3) |
| Average % Recovery |
| 86 |
| Range |
| 83 -89% |
| Sample |
| urine (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
Linearity
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 98% |
| 1:8 | 109% |
| 1:16 | 95% |
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-4 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-4 protein in Cell supernatant . It contains recombinant Human IL-4, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
IL-4 ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL4. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL-4 ELISA Kit, Human: Synonyms
BCGF-1 ELISA Kit, Human; BCGF1 ELISA Kit, Human; BSF-1 ELISA Kit, Human; BSF1 ELISA Kit, Human; IL-4 ELISA Kit, Human; Interleukin-4 ELISA Kit, Human
IL-4 Background Information
Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
Full Name
interleukin 4
Related Pathways
-
Common G-Chain signaling pathway
References
- Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9.
- Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
- Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.
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