IL-32 ELISA Kit, Human

Temporarily not available outside of China.
1/2
Price:
Size:
Number:

IL-32 ELISA Kit, Human: General Information

Product name
IL-32 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
12.1 pg/mL
Assay range
18.75-1200 pg/mL
Specificity
Recognizes both recombinant and natural Human IL-32
Interference
Preparations of C1QB and HSPA8 at 40 ng/mL in a mid-range recombinant human IL32a control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.67 1.33 2.72
SD 0.01 0.05 0.07
CV(%) 2.1% 3.4% 2.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.68 1.33 2.86
SD 0.01 0.03 0.15
CV(%) 1.2% 2.6% 5.3%
Recovery
The recovery of Human IL32 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
81.7528031120264
Range
71-91%
Linearity
Serum
  Recovery of detected
1:2 96%
1:4 102%
1:8 95%
1:16 90%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-32 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-32 protein in Serum . It contains recombinant Human IL-32, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL-32 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural human IL32a. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL-32 ELISA Kit, Human: Synonyms

IL-32alpha ELISA Kit, Human; IL-32beta ELISA Kit, Human; IL-32delta ELISA Kit, Human; IL-32gamma ELISA Kit, Human; IL32 ELISA Kit, Human; NK4 ELISA Kit, Human; TAIF ELISA Kit, Human; TAIFa ELISA Kit, Human; TAIFb ELISA Kit, Human; TAIFc ELISA Kit, Human; TAIFd ELISA Kit, Human

IL-32 Background Information

IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines, and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma and delta) are the most representative IL-32 transcripts, and gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis and in human stomach cancer, human lung cancer and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.
Full Name
interleukin 32
References
  • Felaco P, et al. (2009) IL-32: a newly-discovered proinflammatory cytokine. J Biol Regul Homeost Agents. 23(3): 141-7.
  • Kobayashi H, et al. (2009) Molecular characterization of IL-32 in human endothelial cells. Cytokine. 46(3): 351-8.
  • Meyer N, et al. (2010) IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis. J Allergy Clin Immunol. 125(4): 858-865.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.