IL-16 ELISA Kit, Mouse

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IL-16 ELISA Kit, Mouse: General Information

Product name
IL-16 ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant
Sensitivity
2.97 pg/mL
Assay range
5.17-350 pg/mL
Specificity
Recognizes both recombinant and natural Mouse IL-16
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 38.00 70.00 144.00
SD 1.13 1.69 5.13
CV(%) 3.0% 2.4% 3.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 36.00 75.00 148.00
SD 3.05 2.95 10.50
CV(%) 8.4% 3.9% 7.1%
Recovery
The recovery of Mouse IL16 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
81
Range
78 -86%
Sample
supernatant (n=3)
Average % Recovery
122
Range
115 -126%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
Linearity
Serum
  Recovery of detected
1:2 71%
1:4 85%
1:8 115%
Supernatant
  Recovery of detected
1:2 128%
1:4 106%
1:8 95%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-16 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-16 protein in Serum,Cell supernatant . It contains recombinant Mouse IL-16, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL-16 ELISA Kit, Mouse: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse IL16. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Cross-reactivity was observed to be 0.46% with 50 ng/mL of recombinant human IL16.

IL-16 ELISA Kit, Mouse: Synonyms

mKIAA4048 ELISA Kit, Mouse

IL-16 Background Information

Full Name
interleukin 16
References
  • Maciaszek JW, et al. (1997) IL-16 represses HIV-1 promoter activity. J Immunol. 158(1): 5-8.
  • Zhou P, et al. (1997) Human CD4+ cells transfected with IL-16 cDNA are resistant to HIV-1 infection: inhibition of mRNA expression. Nat Med. 3(6): 659-64.
  • Theodore AC, et al. (1996) CD4 ligand IL-16 inhibits the mixed lymphocyte reaction. J Immunol. 157(5): 1958-64.
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