IL-1 alpha/IL1A ELISA Kit, Mouse

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IL-1 alpha/IL1A ELISA Kit, Mouse: General Information

Product name
IL-1 alpha/IL1A ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant
Sensitivity
3.11 pg/mL
Assay range
6.56-420 pg/mL
Specificity
Recognizes both recombinant and natural Mouse IL-1 alpha/IL1A
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant mouse IL1A control were assayed for interference. No significant interference was observed. Interference was observed with recombinant mouse IL1Ra at concentrations ≥ 0.3125 ng/mL.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 81.00 159.00 317.00
SD 1.77 3.87 7.11
CV(%) 2.2% 2.4% 2.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 79.00 155.00 312.00
SD 3.05 8.29 13.95
CV(%) 3.9% 5.3% 4.5%
Recovery
The recovery of Mouse IL1A spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
93
Range
88 -99%
Sample
supernatant (n=3)
Average % Recovery
101
Range
95 -106%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
Linearity
Serum
  Recovery of detected
1:1 91%
1:2 110%
1:4 103%
1:8 87%
Supernatant
  Recovery of detected
1:1 99%
1:2 102%
1:4 102%
1:8 99%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-1 alpha/IL1A ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-1 alpha/IL1A protein in Serum,Cell supernatant . It contains recombinant Mouse IL-1 alpha/IL1A, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL-1 alpha/IL1A ELISA Kit, Mouse: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse IL1A. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL-1 alpha/IL1A ELISA Kit, Mouse: Synonyms

Il-1a ELISA Kit, Mouse

IL-1 alpha/IL1A Background Information

IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6 and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-1, IL-4, IL-13 and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.
Full Name
interleukin 1, alpha
Research Areas
Related Pathways
  • IL1 signaling pathway
    IL1 signaling pathway
References
  • Nicklin MJ,et al. (1994) A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes. Genomics. 19(2):382-4.
  • March CJ, et al. (1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature. 315(6021):641-7.
  • Bankers-Fulbright JL, et al. (1996) Interleukin-1 signal transduction. Life Sci. 59(2):61-83.
  • Dinarello CA, et al. (1997) Induction of interleukin-1 and interleukin-1 receptor antagonist. Semin Oncol. 24 (3 Suppl 9):S9-81-S9-93.
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