Sino Biological - 官方網站

Hsp27/HSPB1 ELISA Kit, Human

1/2
Price:
Size:
Number:

Hsp27/HSPB1 ELISA Kit, Human: General Information

Product name
Hsp27/HSPB1 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
5.8 pg/mL
Assay range
9.38-600 pg/mL
Specificity
Recognizes both recombinant and natural Human Hsp27/HSPB1
Interference
Preparations of EGED、FTH1、HG3C-MAPKAPK5、HG3C-MAPKAPK3、FC-sMME、TPT1、XRCC5+XRCC6 and CALR-FC at 6 ng/mL in a mid-range recombinant human HSP27 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.15 0.30 0.55
SD 0.00 0.01 0.02
CV(%) 2.8% 3.7% 3.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.11 0.22 0.42
SD 0.01 0.02 0.04
CV(%) 8.3% 8.5% 9.1%
Recovery
The recovery of Human HSPB1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=4)
Average % Recovery
81
Range
78-86%
Linearity
Serum
  Recovery of detected
1:2 112%
1:4 114%
1:8 115%
1:16 106%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This Hsp27/HSPB1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Hsp27/HSPB1 protein in Serum . It contains recombinant Human Hsp27/HSPB1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Hsp27/HSPB1 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human HSP27. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of EGED、FTH1、HG3C-MAPKAPK5、HG3C-MAPKAPK3、FC-sMME、TPT1、XRCC5+XRCC6 and CALR-FC at 6 ng/mL in a mid-range recombinant human HSP27 control were assayed for interference. No significant interference was observed.

Hsp27/HSPB1 ELISA Kit, Human: Alternative Names

CMT2F ELISA Kit, Human; HEL-S-102 ELISA Kit, Human; HMN2B ELISA Kit, Human; HS.76067 ELISA Kit, Human; Hsp25 ELISA Kit, Human; HSP27 ELISA Kit, Human; HSP28 ELISA Kit, Human; SRP27 ELISA Kit, Human

Hsp27/HSPB1 Background Information

Full Name
heat shock 27kDa protein 1
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait