Her2/ERBB2 ELISA Kit, Human

Temporarily not available outside of China.
1/2
Price:
Size:
Number:

Her2/ERBB2 ELISA Kit, Human: General Information

Product name
Her2/ERBB2 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
7 pg/mL
Assay range
62.5-4000 pg/mL
Specificity
Recognizes both recombinant and natural Human Her2/ERBB2
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.42 0.84 1.74
SD 0.01 0.02 0.05
CV(%) 2.4% 2.9% 3.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.46 0.92 1.95
SD 0.04 0.08 0.16
CV(%) 9.0% 9.1% 8.4%
Recovery
The recovery of Human ERBB2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=4)
Average % Recovery
90
Range
86 -92%
Linearity
Serum
  Recovery of detected
1:2 93%
1:4 93%
1:8 93%
1:16 81%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This Her2/ERBB2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Her2/ERBB2 protein in Serum . It contains recombinant Human Her2/ERBB2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Her2/ERBB2 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human ErbB2. The factors listed above were prepared at 1250 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Her2/ERBB2 ELISA Kit, Human: Synonyms

CD340 ELISA Kit, Human; ENV ELISA Kit, Human; ENVW ELISA Kit, Human; ERVWE1 ELISA Kit, Human; HER-2 ELISA Kit, Human; HER-2/neu ELISA Kit, Human; HER2 ELISA Kit, Human; HERV-7q ELISA Kit, Human; HERV-W-ENV ELISA Kit, Human; HERV7Q ELISA Kit, Human; HERVW ELISA Kit, Human; HERVWENV ELISA Kit, Human; MLN 19 ELISA Kit, Human; MLN19 ELISA Kit, Human; NEU ELISA Kit, Human; NGL ELISA Kit, Human; TKR1 ELISA Kit, Human

Her2/ERBB2 Background Information

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Full Name
erb-b2 receptor tyrosine kinase 2
References
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.