Ephrin-A1 ELISA Kit, Human

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Ephrin-A1 ELISA Kit, Human: General Information

Product name
Ephrin-A1 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
4 pg/mL
Assay range
23.44-1500 pg/mL
Specificity
Recognizes both recombinant and natural Human Ephrin-A1
Interference
Preparations of EphA2、EphA3、EphA4 and EphA7 at 15 ng/mL in a mid-range recombinant human EFNA1 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.26 0.47 0.92
SD 0.00 0.01 0.02
CV(%) 1.2% 1.6% 1.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.23 0.46 0.89
SD 0.02 0.01 0.03
CV(%) 7.7% 2.3% 3.7%
Recovery
The recovery of Human EFNA1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
80
Range
74-85%
Linearity
Supernatant
  Recovery of detected
1:2 99%
1:4 103%
1:8 97%
1:16 86%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This Ephrin-A1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Ephrin-A1 protein in Cell supernatant . It contains recombinant Human Ephrin-A1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Ephrin-A1 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human EFNA1.The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Ephrin-A1 ELISA Kit, Human: Synonyms

B61 ELISA Kit, Human; ECKLG ELISA Kit, Human; EFL1 ELISA Kit, Human; EFNA1 ELISA Kit, Human; EPLG1 ELISA Kit, Human; LERK-1 ELISA Kit, Human; LERK1 ELISA Kit, Human; TNFAIP4 ELISA Kit, Human

Ephrin-A1 Background Information

EPH-related receptor tyrosine kinase ligand 1 (abbreviated as Ephrin-A1) also known as ligand of eph-related kinase 1 or EFNA1, is a member of the ephrin (EPH) family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A1/EFNA1 and its Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A1 and one of its receptor EphA2 were expressed in xenograft endothelial cells and also tumor cells and play a role in human cancers, at least in part by influencing tumor neovascularization.
Full Name
ephrin-A1
References
  • Deroanne C, et al. (2003) EphrinA1 inactivates integrin-mediated vascular smooth muscle cell spreading via the Rac/PAK pathway. J Cell Sci. 116(7): 1367-76.
  • Ojima T, et al. (2006) EphrinA1 inhibits vascular endothelial growth factor-induced intracellular signaling and suppresses retinal neovascularization and blood-retinal barrier breakdown. Am J Pathol. 168(1): 331-9.
  • Wu D, et al. (2004) Prognostic value of EphA2 and EphrinA-1 in squamous cell cervical carcinoma. Gynecol Oncol. 94(2): 312-9.
  • EphA2 contributes to disruption of the blood-brain barrier in cerebral malaria
    Author
    Darling, TK;Mimche, PN;Bray, C;Umaru, B;Brady, LM;Stone, C;Eboumbou Moukoko, CE;Lane, TE;Ayong, LS;Lamb, TJ;
    Year
    2020
    Journal
    PLoS Pathog.
    Application
    ELISA
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