Endoglin/CD105 ELISA Kit, Human

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Endoglin/CD105 ELISA Kit, Human: General Information

Product name
Endoglin/CD105 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
36.27 pg/mL
Assay range
62.5-4000 pg/mL
Specificity
Recognizes both recombinant and natural Human Endoglin/CD105
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 834.00 1,647.00 3,158.00
SD 22.58 41.64 95.14
CV(%) 2.7% 2.5% 3.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 827.00 1,662.00 3,209.00
SD 42.17 92.50 184.82
CV(%) 5.1% 5.6% 5.8%
Recovery
The recovery of Human ENG spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
81
Range
79 -83%
Linearity
Serum
  Recovery of detected
1:2 102%
1:4 98%
1:8 117%
1:16 101%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This Endoglin/CD105 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Endoglin/CD105 protein in Serum . It contains recombinant Human Endoglin/CD105, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Endoglin/CD105 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD105. The factors listed below were prepared at 50 ng/mL or 100ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Endoglin/CD105 ELISA Kit, Human: Synonyms

END ELISA Kit, Human; HHT1 ELISA Kit, Human; ORW1 ELISA Kit, Human

Endoglin/CD105 Background Information

Endoglin, also known as CD15, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
Full Name
endoglin
References
  • Bellon T., et al.,(1993), Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23:2340-2345.
  • Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.
  • Gougos A., et al.,(1990), Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.J. Biol. Chem. 265:8361-8364.
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