EGFR ELISA Kit, Human

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EGFR ELISA Kit, Human: General Information

Product name
EGFR ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
3.16 pg/mL
Assay range
15.63-1000 pg/mL
Specificity
Recognizes both recombinant and natural Human EGFR
Interference
Preparations of human EGF at 10 ng/mL in a mid-range recombinant human EGFR control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 119.85 219.35 428.84
SD 2.12 3.84 14.57
CV(%) 1.8% 1.8% 3.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 198.40 357.00 754.60
SD 11.19 27.27 29.56
CV(%) 5.6% 7.6% 3.9%
Recovery
The recovery of Human EGFR spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=4)
Average % Recovery
102
Range
91-115%
Linearity
Serum
  Recovery of detected
1:2 98%
1:4 85%
1:8 79%
1:16 73%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This EGFR ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human EGFR protein in Serum . It contains recombinant Human EGFR, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

EGFR ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human EGFR. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

EGFR ELISA Kit, Human: Synonyms

ERBB ELISA Kit, Human; ERBB1 ELISA Kit, Human; HER1 ELISA Kit, Human; mENA ELISA Kit, Human; NISBD2 ELISA Kit, Human; PIG61 ELISA Kit, Human

EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Full Name
epidermal growth factor receptor
Related Pathways
  • EGFR Signaling Pathway
    EGFR Signaling Pathway
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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