CD38 ELISA Kit, Human

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CD38 ELISA Kit, Human: General Information

Product name
CD38 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Recombinant protein
Sensitivity
18.45 pg/mL
Assay range
39.06-2500 pg/mL
Specificity
Recognizes both recombinant and natural Human CD38
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 535.00 1,059.00 2,047.00
SD 51.40 56.10 96.56
CV(%) 9.6% 5.3% 4.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 553.00 1,111.00 2,097.00
SD 16.70 48.05 55.14
CV(%) 3.0% 4.3% 2.6%
Recovery
The recovery of Human CD38 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
109
Range
109 -110%
Linearity
Supernatant
  Recovery of detected
1:2 102%
1:4 97%
1:8 101%
1:16 90%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CD38 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD38 protein in Recombinant protein . It contains recombinant Human CD38, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD38 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant Human CD38.The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Citation List

CD38 ELISA Kit, Human: Synonyms

ADPRC 1 ELISA Kit, Human; ADPRC1 ELISA Kit, Human; T10 ELISA Kit, Human

CD38 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.
Full Name
CD38 molecule
Research Areas
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Nanobody-based dual epitopes protein identification (DepID) assay for measuring soluble CD38 in plasma of multiple myeloma patients
    Author
    Li, T;Li, S;Fang, C;Hou, Y;Zhang, Q;Du, X;Lee, H;Zhao, Y;
    Year
    2018
    Journal
    Analytica Chimica Acta
    Application
    ELISA (plasma sample)
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