C-Reactive Protein ELISA Kit, Human

Temporarily not available outside of China.
1/2
Price:
Size:
Number:

C-Reactive Protein ELISA Kit, Human: General Information

Product name
C-Reactive Protein ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
21.78 pg/mL
Assay range
54.69-3500 pg/mL
Specificity
Recognizes both recombinant and natural Human C-Reactive Protein
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human CRP control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 516.00 1,012.00 2,061.00
SD 16.29 38.75 100.06
CV(%) 3.2% 3.8% 4.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 520.00 1,039.00 2,047.00
SD 14.45 29.47 19.24
CV(%) 2.8% 2.8% 0.9%
Recovery
The recovery of Human CRP spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
101
Range
99 -104%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
Linearity
Serum
  Recovery of detected
1:1 98%
1:2 98%
1:4 102%
1:8 102%
Supernatant
  Recovery of detected
1:1 93%
1:2 98%
1:4 109%
1:8 95%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This C-Reactive Protein ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human C-Reactive Protein protein in Serum . It contains recombinant Human C-Reactive Protein, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

C-Reactive Protein ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CRP. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

C-Reactive Protein ELISA Kit, Human: Synonyms

C-Reactive Protein ELISA Kit, Human; PTX1 ELISA Kit, Human

C-Reactive Protein Background Information

C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.
Full Name
C-reactive protein, pentraxin-related
References
  • Pepys MB. et al., 2003, J Clin Invest. 111 (12): 1805-12.
  • Thompson D. et al., 1999, Structure. 7(2): 169-77.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.