B4GALT1 ELISA Kit, Human

Temporarily not available outside of China.Online Datasheet is a general version, for batch specific information please refer to the hard copy in shipping package.
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B4GALT1 ELISA Kit, Human: General Information

Product name
B4GALT1 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Lysate
Sensitivity
78.00 pg/mL
Assay range
156.25-10000 pg/mL
Specificity
Recognizes both recombinant and natural Human B4GALT1
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1.94 3.89 7.88
SD 0.06 0.12 0.39
CV(%) 3.2% 3.1% 5.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1.57 3.02 6.28
SD 0.10 0.23 0.58
CV(%) 6.3% 7.5% 9.3%
Recovery
The recovery of Human B4GALT1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
108
Range
105-115%
Linearity
Supernatant
  Recovery of detected
1:2 109%
1:4 108%
1:8 106%
1:16 96%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This B4GALT1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human B4GALT1 protein in Lysate . It contains recombinant Human B4GALT1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

B4GALT1 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human B4GALT1.The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

B4GALT1 ELISA Kit, Human: Synonyms

B4GAL-T1 ELISA Kit, Human; beta4Gal-T1 ELISA Kit, Human; CDG2D ELISA Kit, Human; GGTB2 ELISA Kit, Human; GT1 ELISA Kit, Human; GTB ELISA Kit, Human

B4GALT1 Background Information

Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.
Full Name
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
References
  • Hennet T. (2002) The galactosyltransferase family. Cellular and Molecular Life Sciences. 59(7): 1081-95.
  • Landers EA, et al. (2009) Porcine 1, 4-Galactosyltransferase-I Sequence and Expression. Reproduction in Domestic Animals. 44(2): 228-34.
  • Amado M, et al. (2000) Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim Biophys Acta. 1473 (1): 35-53.
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