The ELISA method is a benchmark for quantitation of pathological antigens and there are indeed many variations to this method. ELISAs are adaptable to high-throughput screening because results are rapid, consistent and relatively easy to analyze. The best results have been obtained with the sandwich format, utilizing highly purified, prematched capture and detector antibodies. The resulting signal provides data which is very sensitive and highly specific.
Linearity range is used for quantitative calculation of sample value. Through measuring a series of absorbance for standard material that is known concentration, and we can get the curve, and then we can calculate the actual testing sample value. Linearity range is crucial for the use of ELISA Kit.