ELISA Antibody-biological target

Background

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate.

Biological target is very critical to drug discovery, drug development and disease diagnosis. Based on published estimates, there are approximately 1100 host biological targets and 200 pathogen biological targets. Biological target reagents can be used in pharmaceutical industry and academic biomedical research for immunomodulation, inflammation, cardiovascular diseases, central nervous system (CNS) and cancer etc.