Elisa Antibody-Stem Cell Research

Background

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate.

Stem Cells are characterised by their two properties of capability of renewing themselves and differentiating into a diverse range of cell types. Stem cell related antibody is an important means in the study of stem cells, stem cell related antibody provides the effective and fast method for stem cell separation, stem cell purification and identification of stem cell marker. The use of different stem cell related growth Factors, chemokines and cytokines in particular conditions on stem cells were induced toward a particular cell differentiation.