Elisa Antibody-Mouse Monoclonal Antibody


Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate.

Mouse monoclonal antibodies are produced by one type of immune cell and are all clones of a single parent cell. Monoclonal antibodies have monovalent affinity, in that they bind to the same epitope. Currently, Mouse monoclonal antibody technology is so valuable, not only can antibodies be used to protect against disease, they can also help to detect the presence of drugs, viral and bacterial products, and other unusual or abnormal substances in the blood. This has become an important tool in biochemistry, molecular biology and medicine.