Elisa Antibody-Interkeukine

Background

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate.

Interleukins are a type of cytokines that were first seen to be expressed by leukocytes. The term 'interleukin' describe a number of secreted molecules produced by leukocytes, a variety of polypeptides that act specifically as mediators between leucocytes. . Interleukin (IL) has been used to describe a group of cytokines with complex immunomodulatory functions - including cell proliferation, maturation, migration and adhesion.