Elisa Antibody-Biomarker

Background

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate.

Biomarker (or biological marker) is a substance which exists in a living organism; which usually is used to measure the transformation of biological system, organ, tissue, subcellular structure or their function. Biomarkers are used in many scientific fields.Biomarkers are also measured the diagnosis of disease, dividing the stage of disease, which can be used to evaluate the effect and safety of the new pharmacy and therapy on human.In medicine, a biomarker can be a traceable marker that is injected into an organism as a means to evaluate organ's function or other aspects of health.