Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP Antibody, Rabbit PAb, Antigen Affinity Purified

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Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal antibody) General Information

Product name
Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP Antibody, Rabbit PAb, Antigen Affinity Purified
Validated applications
WB,IHC-P,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
EBOV Ebola virus Glycoprotein
Has cross-reactivity in WB with
Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein / GP Protein (His Tag)(40442-V08B1)
Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) Glycoprotein / GP Protein
Ebola virus EBOV (Subtype Sudan, strain Gulu) Glycoprotein / GP Protein (aa:Met1-Asn637, His Tag)(40094-V08B1)
Immunogen
Recombinant Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP Protein (Catalog#40304-V08B1)
Preparation
Produced in rabbits immunized with purified, recombinant Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP (Catalog#40304-V08B1; AAC54887.1; Met1-Gln650), and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal antibody) Images

Anti-Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP rabbit polyclonal antibody at 1:5000 dilution

Lane A:Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein / GP at 10ng

Lane B:GP (B-EBOV-2007)(Cat#40368-V08B1) at 10ng

Lane C:Ebola virus EBOV (subtype Zaire, strain Mayinga 1976) Glycoprotein / GP at 10ng

Lane D:Ebola virus EBOV (Subtype Sudan, strain Gulu) Glycoprotein / GP Protein (aa:Met1-Asn637) at 10ng

Secondary

HRP Conjugated Goat-anti-Rabbit IgG (H+L) at 1/1000 dilution

Performed under reducing conditions.

Ebola virus Glycoprotein Background Information

The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD); the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry; the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
References
  • Volchkov VE, et al. Processing of the Ebola virus glycoprotein by the proprotein convertase furin. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5762-7.
  • Lee JE, et al. Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor. Nature. 2008 Jul 10;454(7201):177-82. doi: 10.1038/nature07082.
  • Hood CL, et al. Biochemical and structural characterization of cathepsin L-processed Ebola virus glycoprotein: implications for viral entry and immunogenicity. J Virol. 2010 Mar;84(6):2972-82. doi: 10.1128/JVI.02151-09.
  • Cook JD and Lee JE. The secret life of viral entry glycoproteins: moonlighting in immune evasion. PLoS Pathog. 2013 May;9(5):e1003258. doi: 10.1371/journal.ppat.1003258.
  • Miller EH and Chandran K. Filovirus entry into cells - new insights. Curr Opin Virol. 2012 Apr;2(2):206-14. doi: 10.1016/j.coviro.2012.02.015.

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