Custom Recombinant Protein Production Service

Why choose us for protein production service ?

• Over 10 years' experience for 6000+ recombinant proteins production.
• One-stop service from gene to protein.
• Multiple protein expression systems: bacterial, yeast, baculovirus-insect and mammalian expression system.
• Multiple purification systems (30+) to choose.
• Abundant R & D ability with over 1000 new proteins per year and quick troubleshooting.
• Close to 100 bioreactors with various volumes ranging from 2 to 1000 L to meet high-throughput and large-scale production.

NOTE: This video displays in Chinese.

All protein production service

  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Expression evaluation
    • Protein expression and purification
    • Large-scale expression and purification (optional)
  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Expression and purification
    • Large-scale expression and purification (optional)
  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Vector linearization and transformation
    • Expression and purification
    • Large-scale expression and purification (optional)
  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Virus packaging and amplification
    • Expression and purification
    • Large-scale expression and purification (optional)
  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Transient expression evaluation (CHO stable cell line development only)
    • Stable cell line development/construction
    • Large-scale expression and purification (optional)

Case Studies of Recombinant Protein Production Service

Case study of E.coli protein A expression     More details>>>

  • SEC-HPLC >98.5%
  • Cell bioactivity: EC50=17pg/mL

Case study of Baculovirus-Insect protein A expression     More details>>>

  • SEC-HPLC >93%
  • Cell bioactivity: EC50=7ng/mL

Case study of HEK293 transient protein A expression     More details>>>

  • SEC-HPLC >98.2%
  • SDS-PAGE >98.7%

Custom Recombinant Protein Production Service Advantages

E.coli protein expression service • Over 10 years' experience of protein expression
• Multiple protein purification and refolding technologies
• High-throughput and large-scale protein expression and production
• One-stop service from gene to purified protein
• Multiple expression vectors and E.coli cells to meet various expression needs
Mammalian transient protein expression service • High-efficiency expression vectors
• Optimized mammalian expression systems
• High-throughput and large-scale production
• High yield expression with post-translational modification and correct folding
• One-stop service from gene sequence to purified protein
Yeast protein expression service • Over 10 years' experience of protein expression
• One-stop service from gene to purified protein
• Efficient expression vectors with high yield
• High-throughput and large-scale protein production
Baculovirus-Insect Cell Protein Expression Service • High-efficiency expression of intracellular and extracellular proteins
• Multiple insect cells: Sf9,Sf21,Hi-5
• High-throughput and large-scale protein production
• Optimized expression vectors and higher-titre virus packaging
Stable cell line development service (CHO / HEK293) • Abundant stable cell line development experience
• Effectively increase stable cell line productivity and improve experimental results
• Integrating all the experimental steps, we provide one-stop service from gene sequence to delivered stable cell line.

Recombinant Protein Production Systems Reference

Expression systems E.coli Yeast Baculovirus-insect Mammalian
prokaryotic eukaryotic eukaryotic eukaryotic
Advantages • low cost
• rapid expression
• easy scale-up
• most widely used system for recombinant protein production
• low cost
• rapid expression
• high yield
• diverse post-translational modifications
• high capacity genes
• soluble proteins
• suitable for toxic proteins
• post-translational modifications similar to mammalian systems
• soluble protein
• lower endotoxin
• better bioactivity
• comprehensive post-translational modifications
• transient & stable expression
Challenges • inclusion bodies
• lack of eukaryotic post-translational modifications
• difficult to express higher MW proteins
• improper glycosylation
• high mannose modification
• more demanding culture conditions
• lack of partial glycosylation
• more demanding culture conditions
Most common applications • bacterial proteins
• antigen proteins
• cytokines
• enzymes
• cytokines
• lower MW proteins
• enzymes
• cytoplasmic proteins
• toxic proteins
• transmembrane proteins
• secretory proteins
• kinases
• secretory proteins
• extracellular domain of transmembrane proteins
• recombinant antibodies
• antibody fragments

Contact Us

Tel
+86-10-58628216; +86-10-58628223
Email
cro-service@sinobiological.com