Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb

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Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb (Rabbit Monoclonal antibody) General Information

Product name
Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb
Validated applications
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
CMV CMV Glycoprotein B
Immunogen
Recombinant Human CMV gB / Glycoprotein B protein (Catalog#10202-V08H1)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CMV gB (Catalog#10202-V08H1; AAA45920.1) extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907).
Source
Monoclonal Rabbit IgG Clone #38
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human cytomegalovirus (HCMV) Glycoprotein B / gB Antibody, Rabbit MAb (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect Human CMV gB in WB. Using a DAB detection system, the detection limit for Human CMV gB is approximately 0.125 ng/lane under non-reducing conditions and 100 ng/lane under reducing conditions.
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

CMV Glycoprotein B Background Information

Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
References
  • Lopper M,et al. (2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82.
  • Isaacson MK, et al. (2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.

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