PCSK9 cDNA ORF Clone, Human, C-GFPSpark® tag
Cat: HG15813-ACG
PCSK9 cDNA ORF Clone, Human, C-GFPSpark® tag: General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
2079 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1026A>G, 1380A>G not causing the amino acid variation.
Description
Full length Clone DNA of Human proprotein convertase subtilisin/kexin type 9 with C terminal GFPSpark tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
Restriction Sites
KpnI (two restriction sites) + XbaI (6kb+2.35kb+0.5kb)
Tag Sequence
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
PCSK9 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
PCSK9 cDNA ORF Clone, Human, C-GFPSpark® tag: Validated Images
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.
PCSK9 cDNA ORF Clone, Human, C-GFPSpark® tag: Synonyms
FH3 cDNA ORF Clone, Human; HCHOLA3 cDNA ORF Clone, Human; LDLCQ1 cDNA ORF Clone, Human; NARC-1 cDNA ORF Clone, Human; NARC1 cDNA ORF Clone, Human; PC9 cDNA ORF Clone, Human
PCSK9 Background Information
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Full Name
proprotein convertase subtilisin/kexin type 9
Research Areas
References
- Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
- Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498
- Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
- Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
- Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
PCSK9 cDNA ORF Clone, Human, Related Products
PCSK9 cDNA ORF Clone, Mouse, Related Products
PCSK9 cDNA ORF Clone, Rat, Related Products
If you couldn't find the specific citation, you can:
