CD59 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag

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CD59 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: General Information

Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
387 bp
Description
Full length Clone DNA of Human CD59 molecule, complement regulatory protein with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

CD59 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: Alternative Names

16.3A5 cDNA ORF Clone, Human; 1F5 cDNA ORF Clone, Human; EJ16 cDNA ORF Clone, Human; EJ30 cDNA ORF Clone, Human; EL32 cDNA ORF Clone, Human; FLJ38134 cDNA ORF Clone, Human; FLJ92039 cDNA ORF Clone, Human; G344 cDNA ORF Clone, Human; HRF-20 cDNA ORF Clone, Human; HRF20 cDNA ORF Clone, Human; MAC-IP cDNA ORF Clone, Human; MACIF cDNA ORF Clone, Human; MEM43 cDNA ORF Clone, Human; MGC2354 cDNA ORF Clone, Human; MIC11 cDNA ORF Clone, Human; MIN1 cDNA ORF Clone, Human; MIN2 cDNA ORF Clone, Human; MIN3 cDNA ORF Clone, Human; MIRL cDNA ORF Clone, Human; MSK21 cDNA ORF Clone, Human; p18-20 cDNA ORF Clone, Human

CD59 Background Information

CD59 glycoprotein, also known as 2 kDa homologous restriction factor, HRF2, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
Full Name
CD59 molecule
References
  • Fletcher CM. et al., 1994, Structure. 2: 185-99.
  • Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44.
  • Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81.
  • Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9.
  • Picariello G. et al., 2008, Proteomics 8: 3833-47.
  • Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.
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