Anti-CD3 epsilon / CD3e Antibody (FITC)

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Anti-CD3 epsilon / CD3e Antibody (FITC) (Mouse Monoclonal antibody) General Information

Product name
Anti-CD3 epsilon / CD3e Antibody (FITC)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human CD3 epsilon / CD3e
Immunogen
Recombinant Human CD3e / CD3 epsilon protein (Catalog#10977-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3e / CD3 epsilon (rh CD3e / CD3 epsilon; Catalog#10977-H08H; NP_000724.1; Met1-Asp126) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Mouse IgG2a Clone #001
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
FITC
Concentration
5 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-CD3 epsilon / CD3e Antibody (FITC) (Mouse Monoclonal antibody) Images

Flow cytometric analysis of Human CD3 expression on human peripheral blood lymphocytes. Cells were stained with FITC-conjugated anti-Human CD3 and APC conjugated anti-Human CD19 (BD Pharmingen™ Cat. No. 555415). The dot plots were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.

Anti-CD3 epsilon / CD3e Antibody (FITC): Alternative Names

Anti-CD3 epsilon Antibody; Anti-IMD18 Antibody; Anti-T3E Antibody; Anti-TCRE Antibody

CD3 epsilon / CD3e Background Information

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.
Full Name
CD3e molecule, epsilon (CD3-TCR complex)
References
  • Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
  • Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
  • Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
  • Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.

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