CD27 Protein, Human, Recombinant (His & Fc Tag)

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CD27 Protein, Human, Recombinant (His & Fc Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its ability to bind with recombinant human CD70 . Immobilized recombinant human CD27 at 2 μg/ml (100 μl/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Ile 192) of human CD27 (NP_001233.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the carboxy-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Thr 21
Molecule Mass
The recombinant mature human CD27/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 420 amino acids and has a calculated molecular mass of 47.2 kDa. As a result of glycosylation, the monomer migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD27 Protein, Human, Recombinant (His & Fc Tag): Images

Measured by its ability to bind with recombinant human CD70 . Immobilized recombinant human CD27 at 2 μg/ml (100 μl/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml

CD27 Protein, Human, Recombinant (His & Fc Tag): Alternative Names

S152 Protein, Human; S152. LPFS2 Protein, Human; S152.LPFS2 Protein, Human; T14 Protein, Human; TNFRSF7 Protein, Human; Tp55 Protein, Human

CD27 Background Information

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD7 on lymphocytes and dendritic cells. The CD27-CD7 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.
Full Name
CD27 molecule
Research Areas
References
  • Drner T, et al. (2004) Correlation of circulating CD27 high plasma cells and disease activity in systemic lupus erythematosus. Lupus. 13(5): 283-9.
  • Sahota SS, et al. (2009) CD27 in defining memory B-cell origins in Waldenstrm's macroglobulinemia. Clin Lymphoma Myeloma. 9(1): 33-5.
  • Jiang J, et al. (2010) Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis. J Clin Immunol. 30(4): 566-73.
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