Anti-CD20 Antibody
Cat: 11007-MM01
Cat: 11007-MM01
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Anti-CD20 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD20 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD20
Immunogen
Recombinant Human CD20 / MS4A1 Protein (Catalog#11007-H07H2)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD20 / MS4A1 (rh CD20 / MS4A1 ; Catalog#11007-H07H2; NP_068769.2; Glu213-Pro297). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #01
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-CD20 Antibody (Mouse Monoclonal antibody) Validated Applications
| Application | Dilution |
|---|---|
| ELISA | 1:1000-1:2000 |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-CD20 Antibody: Alternative Names
Anti-B1 Antibody; Anti-Bp35 Antibody; Anti-CD20 Antibody; Anti-CVID5 Antibody; Anti-LEU-16 Antibody; Anti-MS4A1 Antibody; Anti-MS4A2 Antibody; Anti-S7 Antibody
CD20 Background Information
CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed on all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. CD20 / MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.
Full Name
membrane-spanning 4-domains, subfamily A, member 1
Research Areas
- Immunology>>Adaptive Immunity>>B Cell>>B Cell CD Antigen
- Stem Cells>>Cancer Stem Cells (CSCs)>>Cancer Stem Cell (CSC) Marker
- Stem Cells>>Hematopoietic Stem Cells (HSCs)>>Hematopoietic Stem Cell Negative Marker
- Stem Cells>>Hematopoietic Stem Cells (HSCs)>>Human Lineage Negative
- Cancer>>Cancer Types>>Lymphoma
- Neuroscience>>Neurology process>>Neurodegeneration and Neurodegenerative Disease>>Multiple sclerosis
- Cancer>>Cancer immunology>>Tumor-associated antigen
References
- Tedder TF, et al. (1988) Isolation and structure of a cDNA encoding the B1 (CD20) cell-surface antigen of human B lymphocytes. Proc Natl Acad Sci. 85(1): 208-12.
- Cragg MS, et al. (2005) The biology of CD20 and its potential as a target for mAb therapy. Curr Dir Autoimmun. 8: 140-74..
- Polyak MJ, et al. (2003) A cholesterol-dependent CD20 epitope detected by the FMC7 antibody. Leukemia. 17(7): 1384-9.
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