CD11b/ITGAM/Integrin alpha M Protein

CD11b/ITGAM/Integrin alpha M Protein Overview

CD11b/ITGAM/Integrin alpha M reagents

A major surface antigen family on human leukocytes includes complement receptor type 3 (CR3A; also called ITGAM, Mac1, or Mo1), lymphocyte function-associated (LFA) antigen type 1 (ITGAL; 153370), and p150,95 (ITGAX; 151510). These antigens share a common beta chain (ITGB2; 600065) of 94 kD, linked noncovalently to 1 of 3 alpha chains distinctive to each. They promote adhesion of granulocytes to each other and to endothelial cell monolayers. The apparent molecular weight of the Mo1 alpha chain is 155 to 165 kD, that of the LFA1 alpha subunit is 180 kD, and that of the Leu M5 subunit is 130 to 150 kD. Pierce et al. (1986) purified human Mo1 to homogeneity from normal granulocytes by affinity chromatography and high performance liquid chromatography (HPLC) and determined the N-terminal amino acid sequence of its alpha subunit. The obtained sequence was identical, except for 2 conservative substitutions, to that of the alpha subunit of Mac1 antigen (Springer et al., 1985). Furthermore, Pierce et al. (1986) found that the N-terminal amino acid sequence of the alpha subunit of Mo1 was homologous to the alpha subunit of IIb/IIIa (607759), a glycoprotein that serves similar adhesive functions on platelets and is deficient or defective in Glanzmann thrombasthenia (273800). Patients with a history of recurrent bacterial infections and an inherited deficiency of all 3 leukocyte membrane surface antigens are thought to have reduced or absent synthesis of the common beta subunit of the antigen family; see 116920. Arnaout et al. (1988) described the isolation and analysis of 2 partial cDNA clones encoding the alpha subunit of Mo1 in humans and guinea pigs. A comparison of the coding region of the MO1A gene revealed significant homology with the carboxyl-terminal portions of the alpha subunits of fibronectin, vitronectin, and platelet IIb/IIIa receptors. Corbi et al. (1988) described full-length cDNA clones for the alpha subunit of Mac1. Arnaout et al. (1988) reported the complete amino acid sequence as deduced from cDNA for the human alpha subunit. The protein consists of 1,136 amino acids with a long amino-terminal extracytoplasmic domain, a 26-amino acid hydrophobic transmembrane segment, and a 19-carboxyl-terminal cytoplasmic domain. The alpha subunit is highly similar in sequence to the alpha subunit of leukocyte p150,95.

CD11b/ITGAM/Integrin alpha M protein family

Belongs to the integrin alpha chain family.

CD11b/ITGAM/Integrin alpha M protein name

Recommended name
Integrin alpha-M
Aliases
CD11b, complement component 3 receptor 3 subunit, MAC-1

CD11b/ITGAM/Integrin alpha M Protein Molecular Weight & PI

The parameters have been computed for the following feature

FT CHAIN 17-1152 Integrin alpha-M.

Molecular weight (Da)

125471.34

Theoretical pI

6.75

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