Neuraminidase/NA Proteins, Antibodies, cDNA Clones Research Reagents

Influenza neuraminidase (NA) is a type of neuraminidase. The neuraminidase activity of NA protein plays a major role in the release of nascent viral particles assembled in infected cells. Small molecules that inhibit neuraminidase activity will block the release of newly assembled particles and thereby progeny virus will not spread to neighboring cells. Influenza NA is composed of four identical subunits arranged in a square. Neuraminidase enzymatic activity is independent of sucrose fermentation, isolation site, and hemagglutination.

Neuraminidase/NA Protein (76)

Neuraminidase/NA Antibody (15)

    Neuraminidase/NA cDNA Clone (207)

    A/Egypt/2321-NAMRU3/2007
    A/Thailand/1(KAN-1)/2004
    A/Anhui/1/2005

    In expression vector

    In lentiviral vector

    A/green-winged teal/ALB/199/1991
    A/mallard/Ohio/657/2002
    A/Hong Kong/1073/99
    A/Chicken/Hong Kong/G9/1997
    A/mallard duck/Alberta/299/1977
    A/breeder duck/Korea/Gochang1/2014
    A/canine/New York/145353/2008
    A/duck/Guangdong/E1/2012
    B/PHUKET/3073/2013

    In expression vector

    In lentiviral vector

    A/duck/Guangdong/E1/2012

    In cloning vector

    A/Jiangxi-Donghu/346/2013

    In expression vector

    In lentiviral vector

    A/duck/Hokkaido/167/2007
    A/Babol/36/2005
    A/Switzerland/9715293/2013

    In expression vector

    A/Hong Kong/4801/2014

    In expression vector

    A/Memphis/1/68

    In expression vector

    In lentiviral vector

    A/Aichi/2/1968

    In expression vector

    In lentiviral vector

    A/Anhui/1/2013
    A/Shanghai/1/2013

    In lentiviral vector

    A/USSR/90/1977

    In expression vector

    In lentiviral vector

    A/California/04/2009

    In lentiviral vector

    A/Puerto Rico/8/1934

    In expression vector

    In lentiviral vector

    A/Netherlands/219/2003

    In expression vector

    In lentiviral vector

    Neuraminidase/NA Lysate (51)

      Neuraminidase/NA Background

      Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.

      Neuraminidase/NA References

      • Sardet C., et al.,(1989), Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter. Cell 56:271-280.
      • Sardet C., et al., (1990), Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.Science 247:723-726.
      • Tse C.-M., et al.,(1991), Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.EMBO J. 10:1957-1967.

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