Myc tag Antibodies Research Reagents

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. The peptide sequence of the myc-tag is Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu. The tag is approximately 1202 Da and has 10 amino acids. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Myc tag Antibody (3)

    Myc tag Background

    The Myc tag is a popular protein tag derived from c-myc gene product with a molecular weight of 1.2 KDa. The amino acid sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. Anti-Myc Tag antibodies can facilitate the detection, separation and purification of Myc tag target proteins without protein-specific antibodies or probes in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques.

    Myc tag References

    • Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
    • Hillman MC, et al. (2001) A comprehensive system for protein purification and biochemical analysis based on antibodies to c-myc peptide. Protein Expr Purif 23 (2): 359-368.
    • Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.

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