MLK3 Antibodies, cDNA Clones Research Reagents

MAP3K11 (Mitogen-Activated Protein Kinase Kinase Kinase 11, also known as MLK3; PTK1; SPRK; MLK-3; MEKK11), located on 11q13.1, is a Protein Coding gene. The encoded protein belongs to the STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily. Its structural features include an SH3 domain in the absence of an SH2 domain, a region containing 2 leucine zippers with an adjacent C-terminal basic region, and a proline-rich region. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of the JNK signaling pathway.

MLK3 Antibody (2)

    MLK3 cDNA Clone (15)

    MLK3 qPCR Primer (1)

    MLK3 Background

    Mixed-lineage kinase 3 (MLK3; also known as MAP3K11) is a Ser/Thr protein kinase widely expressed in normal and cancerous tissues, including brain, lung, liver, heart, and skeletal muscle tissues. Its Src homology 3 (SH3) domain has been implicated in MLK3 autoinhibition and interactions with other proteins, including those from viruses. The MLK3 SH3 domain contains a six-amino-acid insert corresponding to the n-Src insert, suggesting that MLK3 may bind additional peptides. MAP3K11 was initially identified in a megakaryocytic cell line and is an emerging therapeutic target in cancer. MAP3K11 directly phosphorylates JNKs and may control activation of ERKs. MAP3K11 is a tightly regulated MAP3K member. Consistent with its known role in the activation of the AP-1 pathway through JNK kinase, MLK3 was able to enhance Tat-dependent HIV transcription in vitro thus leading to an increase in infection signal.

    MLK3 References

    • Nguyen DG, et al. (2007) Identification of novel therapeutic targets for hiv infection through functional genomic cdna screening. Virology 362 (1): 16-25.
    • Kokoszka ME, et al. (2018) Identification of two distinct peptide-binding pockets in the sh3 domain of human mixed-lineage kinase 3. J Biol Chem 293 (35): 13553-13565.
    • Gibbs GM, et al. (2007) Cysteine-rich secretory protein 2 binds to mitogen-activated protein kinase kinase kinase 11 in mouse sperm. Biol Reprod 77 (1): 108-114.

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