Immunohistochemistry, using the basic principles of immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides and proteins) in tissues, its location, qualitative and quantitative research, called immunohistochemistry (immunohistochemistry) or immunocytochemistry techniques (immunocytochemistry).It combined the specific of immune response with visibility of histochemistry by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, detection of various antigens in a cell, subcellular level, such as proteins, polypeptides, enzymes, hormones, pathogens, and receptors. Immunohistochemical techniques have developed rapidly in recent years. It was limited to immunofluorescence techniques in 1950s, but gradually developed after the 1950s to establish a highly sensitive and more practical immunization enzyme technology.
Frozen sections is a slicing method that the tissue was rapidly cooled to a certain hardness at low temperature and then sliced. Because the production process of frozen sections is fast and easy than the paraffin and more used in rapid pathological diagnosis of surgery.
Cytoplasm staining as follows:
|EGFR / HER1 / ErbB1 : 10001-R043
Immunofluorescence staining of EGFR in monkey skin with rabbit monoclonal antibody (20 μg/mL, frozen section). The image showing membrane staining of squamous epithelium cell.
|E-Cadherin / CDH1 / E-cad / CD324: 10204-RP02
Immunofluorescence staining of human E-cadherin in human skin with Rabbit Polyclonal Antibody (5 µg/mL, frozen sections). An DyLight488 goat anti-rabbit antibody was used as the secondary antibody. The image showing membrane staining of squamous epitheliu
|CD147 / EMMPRIN / Basigin: 10186-R125
Immunofluorescence staining of CD147 in monkey stomach with rabbit monoclonal antibody (1μg/mL, frozen section). The image showing membrane staining of gastric gland cell. The right panel: merge with DAPI