Immunohistochemistry, using the basic principles of immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides and proteins) in tissues, its location, qualitative and quantitative research, called immunohistochemistry (immunohistochemistry) or immunocytochemistry techniques (immunocytochemistry).It combined the specific of immune response with visibility of histochemistry by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, detection of various antigens in a cell, subcellular level, such as proteins, polypeptides, enzymes, hormones, pathogens, and receptors. Immunohistochemical techniques have developed rapidly in recent years. It was limited to immunofluorescence techniques in 1950s, but gradually developed after the 1950s to establish a highly sensitive and more practical immunization enzyme technology.
The epididymis is a long, coiled tube that stores sperm and transports it from the testes. The epididymis can be divided into three main regions: the head, the body and the tail. The head of the epididymis receives spermatozoa via the efferent ducts of the mediastinium of the testis. Here the concentration of the sperm is dilute. The tail is involved in absorbing fluid to make the sperm more concentrated. The major role is storage of sperm and ejaculant. A variety of target molecules expressed on epididymis tissue is used for the clinical diagnosis of epididymis disease, epididymis tissue is commonly used in immunohistochemical detection. IHC is an important means to be widely used to detect epididymis disease.
Different species of epididymis slices as follows:
Immunochemical staining of human DDX4 in human epididymis with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of human PPIL1 in human epididymis with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of human NPC2 in human epididymis with rabbit polyclonal antibody (0.05 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of rat LCN2 in rat epididymis with rabbit polyclonal antibody (1µg/mL, formalin-fixed paraffin embedded sections).