Fluorescence Proteins

GFPSpark® is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSpark is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity.

Figure 1. GFP (GFPSpark®) or EGFP expression in Hela cell

Figure 2. GFP (GFPSpark®) tag fusion expression

OFPSpark® is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark is more than twice brighter than mOrange2. Fast OFPSpark maturation makes it clearly detectable in mammalian cells as early as within 8hrs after transfection.

Figure 3. OFP(OFPSpark®) tag fusion expression in Hela cell

Figure 4. OFPSpark® or Commercial orange Red expression in 293 H cell

Part of Featured Fluorescence Protein

Catalogue Product name Purity Expression Host
13105-S07E Aequorea victoria GFP Protein (His Tag) > 90 % as determined by SDS-PAGE Escherichia coli (E. coli)
69001-S08E Aequorea victoria Green fluorescent protein /enhanced GFP GFPSpark® (His Tag) > 97 % as determined by SDS-PAGE Escherichia coli (E. coli)
69002-S08E Discosoma sp. orange / red fluorescent protein / OFP / RFP OFPSpark® (His Tag) > 99 % as determined by SDS-PAGE Escherichia coli (E. coli)

Fluorescent Protein Applications

Almost all endogenous proteins of interest can be fused to fluorescent proteins . Fluorescent proteins as genetically encoded tools are used extensively by life scientists.The main applications of fluorescent proteins include assay promoter activity,organelle marker,protein interaction,drug screening,drganism labeling,sensors.

Organelle Marker

The study of protein subcellular localization is the necessary method of protein function.There are a variety of methods for determining subcellular localization. One of the most common is to use fluorescence microscopy to compare the localization of your protein of interest with fluorescent proteins . Sino Biological has constructed expression-ready vectors which allow N- or C-terminal fusion of the GFPSpark/ OFPSpark gene to a gene of interest allowed the generation of fusion proteins whose subcellular localization was followed by fluorescence microscopy in mammalian cells. Over 1,600 expression ready ORF clones are in-house.

Part of the Product List

Organelle Gene Catalog#  
Cell membrane PDCD1 HG10377-ACG HG10377-ACR
Cell membrane CD274 HG10084-ACG HG10084-ACR
Nucleus HMGB1 HG10326-ACG HG10326-ACR
Nucleus FTO HG12125-ACG HG12125-ACR
Cytoplasm LGALS3 HG10289-ACG HG10289-ACR
Cytoplasm GSK3B HG10044-ACG HG10044-ACR
cytoskeleton SIRT2 HG10830-ACG HG10830-ACR
cytoskeleton TUBA8 HG12013-ACG HG12013-ACR
Mitochondrion TOMM20 HG15094-ACG HG15094-ACR
Mitochondrion HSPD1 HG11322-ACG HG11322-ACR
Mitochondrion BCL2L2 HG10059-ACG HG10059-ACR
Lysosome SCARB2 HG11063-ACG HG11063-ACR
Lysosome CTSC HG10484-ACG HG10484-ACR
Lysosome LAMP3 MG50785-ACG MG50785-ACR
Endoplasmic reticulum HSPA5 HG12063-ACG HG12063-ACR
Endoplasmic reticulum PTPN1 HG10304-ACG HG10304-ACR
Endoplasmic reticulum POR HG10365-ACG HG10365-ACR
Golgi BECN1 HG11162-ACG HG11162-ACR
Golgi GOLM1 HG13066-ACG HG13066-ACR
Microsome PTGS2 HG12036-ACG HG12036-ACR
Endosome RAB5 HG14013-ACG HG14013-ACR
Peroxisome IDH1 HG12055-ACG HG12055-ACR

Background of Fluorescent Protein

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and gene was first cloned in 1992. The first demonstration of its utility as a fluorescent tag for in vivo labeling in 1994,and since then a keen interest in the structure, biochemistry, and biophysics of GFP-like fluorescent proteins, which resulted in an avalanche of scientific publications on FPs and their applications to solve basic problems in molecular and cell biology. Sinobio has self-developed Green fluorescent protein GFPSpark and Red (orange) fluorescent protein OFPSpark.