BTN3A1 Protein, Human, Recombinant (His Tag), Biotinylated

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BTN3A1 Protein, Human, Recombinant (His Tag), Biotinylated: Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human BTN3A1 (NP_919423.1) (Met1-Gly254) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 30
Molecule Mass
The recombinant human BTN3A1 consists of 236 amino acids and predicts a molecular mass of 25.6 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

BTN3A1 Protein, Human, Recombinant (His Tag), Biotinylated: Images

BTN3A1 Protein, Human, Recombinant (His Tag), Biotinylated: Alternative Names

BT3.1 Protein, Human; BTF5 Protein, Human; BTN3.1 Protein, Human; CD277 Protein, Human

BTN3A1 Background Information

BTN3A1 has the structure of a type I receptor of the Ig superfamily and is part of a family of seven BTN receptors encoded by genes in the MHC. BTN molecules are composed of two Ig domains (IgV, IgC2), a single transmembrane domain, and a large carboxyl-terminal domain termed B3.2 (or PRYSPRY) located in the cell cytoplasm. There are three human BTN3A loci, BTN3A1, BTN3A2, and BTN3A3, and clear orthologs of BTN3A molecules, now called CD277, are absent from the mouse genome. Despite its similarity to B7 molecules, BTN3A1 was proposed to act not as a coreceptor or costimulatory molecule, but rather to directly present pAg to the γδ TCR in a manner analogous to MHC-restricted peptide presentation. However, this model of BTN3A1 function has been challenged by conflicting data, which show pAg binding to a positively charged pocket in the cytosolic B3.2 domain, and that BTN3A1 does not directly engage the γδ TCR. This contradictory picture has emerged as a result of the complexity of the system and in particular by the use of endogenous and exogenous routes of Ag delivery in in vitro assays.
Full Name
butyrophilin subfamily 3 member A1
References
  • Rhodes DA, Chen H-C, Price AJ, et al. Activation of human γδ T cells by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin. Journal of immunology (Baltimore, Md : 1950). 2015;194(5):2390-2398.
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