Goat Anti-Rabbit IgG Secondary Antibody (HRP)

 
 
Overview
 

Description:

Goat polyclonal Secondary Antibody to Rabbit IgG (HRP).

Host

Goat

Target species:

Rabbit IgG

Specificity:

By ELISA this antibody reacts specifically with Rabbit IgG.

 

Has cross-reactivity with guinea pig IgG (>10%), rat IgG (2.7%), human IgG (3.6%)

Applications:

WB, ELISA, IHC-P, IP

 
Properties

Form:

Liquid

Storage & Stability:

Protected from prolonged exposure to light. Store at +4℃ short term (1-2 weeks) (Preservative is recommended to avoid contamination). Upon delivery aliquot. Store at -20℃ to -80℃ for twelve months from date of receipt. Avoid repeated freeze-thaw cycles.

Constituents:

PBS

Purification notes:

Immunogen affinity purified

Formulation:

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

 
Applications

The application notes include recommended starting dilutions, optimal dilutions/concentrations should be determined by the end user.

 

IHC-P

0.2-5 μg/mL

ELISA

0.005-0.2 μg/mL

WB

0.02-0.5 μg/mL

(Colormetric: 0.02-0.5 μg/mL; Chemiluminescent: 0.001-0.5 μg/mL)

IP

0.02-0.5 μg/mL

 

Citation List

  • Expression of Programmed cell death protein 1 (PD-1) and Indoleamine 2,3-dioxygenase (IDO) in the tumor microenvironment and in tumor-draining lymph nodes of breast cancer
    Author
    Ye, Q;Wang, C;Xian, J;Zhang, M;Cao, Y;Cao, Y;
    Year
    2018
    Journal
    Hum. Pathol.
    Application
    WB
  • The research progress of p53 tumour suppressor activity controlled by Numb in triple-negative breast cancer
    Author
    Jie, X;Cheng, Y;Xue, Q;Luo, Y;Cao, Y;Cao, Y;
    Year
    2018
    Journal
    bioRxiv
    Application
    WB
  • An EGLN1 mutation may regulate hypoxic response in cyanotic congenital heart disease through the PHD2/HIF-1A pathway
    Author
    Zhou, Y;Ouyang, N;Liu, L;Tian, J;Huang, X;Lu, T;
    Year
    2019
    Journal
    Genes Dis
    Application
    WB
  • The Effects of Hsa-miR-5195-3p on the Proliferation, Migration and Invasion of Human Cervical Cancer SiHa Cells
    Author
    TANG, M;WAN, Q;SUN, S;HU, J;SUN, Z;
    Year
    2020
    Journal
    China Biotechnology
    Application
    WB
  • Progress in the research of p53 tumour suppressor activity controlled by Numb in triple-negative breast cancer
    Author
    Xian, J;Cheng, Y;Qin, X;Cao, Y;Luo, Y;Cao, Y;
    Year
    2020
    Journal
    J. Cell. Mol. Med.
    Application
    WB
  • Cholecystokinin type 2 receptor in colorectal cancer: diagnostic and therapeutic target
    Author
    Chang, J;Liu, ZS;Song, DF;Li, M;Zhang, S;Zhao, K;Guan, YT;Ren, HL;Li, YS;Zhou, Y;Liu, XL;Lu, SY;Hu, P;
    Year
    2020
    Journal
    J. Cancer Res. Clin. Oncol.
    Application
    ICC
  • Human H-ferritin presenting RBM of spike glycoprotein as potential vaccine of SARS-CoV-2
    Author
    Yao, D;Lao, F;Zhang, Z;Liu, Y;Cheng, J;Ding, F;Wang, X;Xi, L;Wang, C;Yan, X;Zhang, R;Ouyang, F;Ding, H;Ke, T;
    Year
    2020
    Journal
    bioRxiv
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