Anti-CD48 Antibody, Rabbit Polyclonal

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Anti-CD48 Antibody, Rabbit Polyclonal General Information

Product name
Anti-CD48 Antibody, Rabbit Polyclonal
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Rat
Specificity
Rat CD48
Immunogen
Recombinant Rat CD48 / SLAMF2 protein (Catalog#80286-R08H)
Preparation
Produced in rabbits immunized with purified, recombinant Rat CD48 / SLAMF2 (Catalog#80286-R08H; P10252; Met1-Arg216). CD48 / SLAMF2 specific IgG was purified by Rat CD48 / SLAMF2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD48 Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:10000-1:50000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD48 Antibody, Rabbit Polyclonal Images

Immunochemical staining of rat CD48 in rat thymus with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).

rat CD48 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

0.5 µL anti-rat CD48 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-rat CD48 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 35 kDa

Anti-CD48 rabbit polyclonal antibody at 1:500 dilution.

Lane A: Jurkat Membrane Lysate

Lysates/proteins at 30 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:48 kDa

(We are unsure as to the identity of these extra bands.)

CD48 Background Information

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
Full Name
CD48 molecule
Research Areas
References
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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