Anti-Myc tag Antibody, Mouse Monoclonal

RRID Number: AB_2860041
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Anti-Myc tag Antibody, Mouse Monoclonal General Information

Product name
Anti-Myc tag Antibody, Mouse Monoclonal
Validated applications
IP
Specificity
Myc tag
Immunogen
A synthetic Myc tag peptide conjugated to KLH
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #07
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Myc tag Antibody, Mouse Monoclonal Validated Applications

Application Dilution
IP 1-4 μg/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Myc tag Antibody, Mouse Monoclonal Images

MYC tag was immunoprecipitated using:

Lane A:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate

Lane B:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate

2 µL anti-MYC tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-MYC tag mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 39 kDa

Observed band size: 39 kDa

Myc tag Background Information

The Myc tag is a popular protein tag derived from c-myc gene product with a molecular weight of 1.2 KDa. The amino acid sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. Anti-Myc Tag antibodies can facilitate the detection, separation and purification of Myc tag target proteins without protein-specific antibodies or probes in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques.
References
  • Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
  • Hillman MC, et al. (2001) A comprehensive system for protein purification and biochemical analysis based on antibodies to c-myc peptide. Protein Expr Purif 23 (2): 359-368.
  • Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.

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Mouse MAb

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