TNFR2/TNFRSF1B/CD120b Neutralizing Antibody

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TNFR2/TNFRSF1B/CD120b Neutralizing Antibody General Information

Product name
TNFR2/TNFRSF1B/CD120b Neutralizing Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Mouse
Specificity
Mouse TNFR2/TNFRSF1B
No cross-reactivity in ELISA with
Human TNFR2 / TNFRSF1B (Catalog#10417-H08H)
Cynomolgus TNFR2 / TNFRSF1B
Immunogen
Recombinant Mouse TNFR2/TNFRSF1B/CD120b protein (Catalog#50128-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TNFR2/TNFRSF1B/CD120b (rM TNFR2/TNFRSF1B/CD120b; Met1-Gly258; NP_035740.2; Catalog#50128-M08H).
Source
Monoclonal Rabbit IgG Clone #R204
Purification
Protein A
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
< 3 EU/mg
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

TNFR2/TNFRSF1B/CD120b Neutralizing Antibody Validated Applications

Notes
Block: In a functional ELISA which immobilized Mouse TNFα (Catalog# 50349-MNAE) at 0.4 μg/mL (100 µL/well) in the plate, the Rabbit anti-Mouse TNFR2/TNFRSF1B Monoclonal Antibody (Catalog# 501528-R204) can block the binding of 0.5 μg/mL of biotinylated Mouse TNFR2/TNFRSF1B (Catalog# 50128-M02H-B) to Mouse TNFα, the EC50 is 0.2-0.7 μg/mL.
Neutralization: The neutralization activity of mouse TNFRSF1B neutralizing antibody is measured by its ability to neutralize mTNFRSF1B-mediated inhibition of cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization Dose(ND50) is typically 16-50 ng/mL in the presence of 25ng/mL mTNFRSF1B,0.5ng/mL mouse TNFa,and 1ug/mL actinomycin D.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

TNFR2/TNFRSF1B/CD120b Neutralizing Antibody Images

Mouse TNFRSF1B Inhibition of Mouse TNFα-induced Cytotoxicity and Neutralization by Mouse TNFRSF1B Antibody. Recombinant Mouse TNFRSF1B (Catalog#50128-M02H) inhibits Recombinant Mouse TNFα(Catalog#50349-MNAE) induced cytotoxicity in the L‑929 mouse fibroblast cell line. Inhibition of Recombinant Mouse TNFα(0.5 ng/mL) activity elicited by Recombinant Mouse TNFRSF1B (25 ng/mL) is neutralized by increasing concentrations of Rabbit Anti-Mouse TNFRSF1B Monoclonal Antibody (Catalog#50128-R204). The ND50 is typically 16-50 ng/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNFR2/TNFRSF1B/CD120b Neutralizing Antibody: Synonyms

Anti-CD120b Antibody; Anti-p75 Antibody; Anti-TNF-alphaR2 Antibody; Anti-TNF-R-II Antibody; Anti-TNF-R2 Antibody; Anti-TNF-R75 Antibody; Anti-TNFalpha-R2 Antibody; Anti-TNFBR Antibody; Anti-Tnfr-1 Antibody; Anti-Tnfr2 Antibody; Anti-TNFR80 Antibody; Anti-TNFRII Antibody

TNFR2/TNFRSF1B Background Information

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Full Name
tumor necrosis factor receptor superfamily, member 1b
Research Areas
References
  • Komata T, et al. (1999) Association of tumor necrosis factor receptor 2 (TNFR2) polymorphism with susceptibility to systemic lupus erythematosus. Tissue Antigens. 53(6): 527-33.
  • Tsuchiya N, et al. (2001) Analysis of the association of HLA-DRB1, TNFalpha promoter and TNFR2 (TNFRSF1B) polymorphisms with SLE using transmission disequilibrium test. Genes Immun. 2(6): 317-22.
  • Guo G, et al. (1999) Role of TNFR1 and TNFR2 receptors in tubulointerstitial fibrosis of obstructive nephropathy. Am J Physiol. 277(5): 766-72.
  • EphB4/ TNFR2/ERK/MAPK signaling pathway comprises a signaling axis to mediate the positive effect of TNF-α on osteogenic differentiation
    Author
    Zhang, Y;Yang, C;Ge, S;Wang, L;Zhang, J;Yang, P;
    Year
    2020
    Journal
    BMC Mol Cell Biol
    Application
    wb&IF

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