Anti-M13 Antibody, Rabbit Polyclonal

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Anti-M13 Antibody, Rabbit Polyclonal General Information

Product name
Anti-M13 Antibody, Rabbit Polyclonal
Validated applications
WB
Species reactivity
Reacts with: other
Specificity
other M13
Immunogen
M13 Bacteriophage coat protein G3P (YP_009111293.1, Ala19-Ala130)
Preparation
Produced in rabbits immunized with purified M13 Bacteriophage coat protein G3P (YP_009111293.1, Ala19-Ala130). phage coat protein g3p of fd phage or M13 phage specific IgG was purified by M13 Bacteriophage coat protein G3P (P69168, Ala19-Ala130) affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-M13 Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:1000-1:5000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-M13 Antibody, Rabbit Polyclonal Images

Anti-NG3P rabbit polyclonal antibody at 1:1000 dilution

Sample:VCSM13 helper phage

Lane A: 1.0E14

Lane B: 1.0E13

Lane B: 1.0E12

Secondary

Goat anti Rabbit IgG (H+L)/HRP at 0.4ug/ml

Performed under reducing conditions.

M13 Background Information

M13 is a filamentous bacteriophage composed of circular single-stranded DNA (ssDNA) which is 647 nucleotides long encapsulated in approximately 27 copies of the major coat protein P8 and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however, the infection causes turbid plaques in E. coli. It is a non-lytic virus. However, a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 5 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 27 copies of p8 to make the coat about 9 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single-stranded genome it packages. The general stages to a viral life cycle are infection, replication of the viral genome, assembly of new viral particles, and then release of the progeny particles from the host. Filamentous phage uses a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single-stranded DNA genome to a double-stranded replicative form.
References
  • Messing, J. et al., 1993, Methods Mol. Biol. 23: 9-22.
  • Mori, K. et al., 1996, Antiviral Res. 31 (1-2): 79-86.
  • Sidhu, S.S. et al., 2001, Biomol Eng. 18 (2): 57-63.
  • Sitohy, M. et al., 2006, J Agric Food Chem. 54 (11): 3800-6.
  • Khalil, A.S. et al., 2007, Proc Natl Acad Sci. USA. 104 (12): 4892-7.
  • A humanized anti-DLL4 antibody promotes dysfunctional angiogenesis and inhibits breast tumor growth
    Author
    Jia, X;Wang, W;Xu, Z;Wang, S;Wang, T;Wang, M;Wu, M;
    Year
    2016
    Journal
    Sci Rep
    Application
    phage display

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