Anti-M13 Antibody (HRP), Mouse Monoclonal

RRID Number: AB_2857928
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Anti-M13 Antibody (HRP), Mouse Monoclonal General Information

Product name
Anti-M13 Antibody (HRP), Mouse Monoclonal
Validated applications
ELISA
Species reactivity
Reacts with: other
Specificity
other M13
Immunogen
M13 Bacteriophage
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with M13 phage. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and then conjugated with Horseradish-peroxidase (HRP).
Source
Monoclonal Mouse IgG1 Clone #MM05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
HRP
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Protected from prolonged exposure to light. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-M13 Antibody (HRP), Mouse Monoclonal Validated Applications

Application Dilution
ELISA 0.1-0.4 μg/mL
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-M13 Antibody (HRP), Mouse Monoclonal Images

Coating: Coat corresponding Protein (20 μg/mL in pH 9.6 carbonate buffer) in NUNC plate
Sample: Add the indicated amounts of M13 Bacteriophages displaying Rabbit/Mouse Fab targeted for different proteins;
Primary Antibody: M13 Antibody (11973-MM05T), 0.365 μg/mL
Secondary Antibody: anti-mouse F(ab)2 Antibody/HRP

M13 Background Information

M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 647 nucleotides long encapsulated in approximately 27 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 5 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 27 copies of p8 to make the coat about 9 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single stranded genome it packages. The general stages to a viral life cycle are: infection, replication of the viral genome, assembly of new viral particles and then release of the progeny particles from the host. Filamentous phage use a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single stranded DNA genome to a double stranded replicative form.
References
  • Messing, J. et al., 1993, Methods Mol. Biol. 23: 9-22.
  • Mori, K. et al., 1996, Antiviral Res. 31 (1-2): 79-86.
  • Sidhu, S.S. et al., 2001, Biomol Eng. 18 (2): 57-63.
  • Sitohy, M. et al., 2006, J Agric Food Chem. 54 (11): 3800-6.
  • Khalil, A.S. et al., 2007, Proc Natl Acad Sci. USA. 104 (12): 4892-7.
  • Identification of a peptide for folate receptor alpha by phage display and its tumor targeting activity in ovary cancer xenograft
    Author
    Xing, L;Xu, Y;Sun, K;Wang, H;Zhang, F;Zhou, Z;Zhang, J;Zhang, F;Caliskan, B;Qiu, Z;Wang, M;
    Year
    2018
    Journal
    Sci Rep
    Application
    ELISA (phage display)
  • Sensitive detection of dengue virus NS1 by highly stable affibody-functionalized gold nanoparticles
    Author
    Bang, J;Park, H;Choi, W;Sung, D;Lee, J;Lee, K;Kim, S;
    Year
    2018
    Journal
    New J. Chem.
    Application
    ELISA (phage display)
  • Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning
    Author
    Kim, ;Lee, ;Park, ;Park, ;Lim, ;So, ;Woo, ;Ko, ;Lee, ;Lim, ;Ko, ;Park, ;Choi, ;Song, ;Lee, ;Kim, ;Kim, ;
    Year
    2019
    Journal
    Antibodies
    Application
    biopanning
  • Production and characterization of a single-chain variable fragment antibody from a site-saturation mutagenesis library derived from the anti-Cry1A monoclonal antibody
    Author
    Dong, S;Gao, M;Bo, Z;Guan, L;Hu, X;Zhang, H;Liu, B;Li, P;He, K;Liu, X;Zhang, C;
    Year
    2020
    Journal
    Int. J. Biol. Macromol.
    Application
    ELISA
  • Blocking antibodies against SARS-CoV-2 RBD isolated from a phage display antibody library using a competitive biopanning strategy
    Author
    Zeng, X;Li, L;Lin, J;Li, X;Liu, B;Kong, Y;Zeng, S;Du, J;
    Year
    2020
    Journal
    bioRxiv
  • Isolation of a human monoclonal antibody specific for the receptor binding domain of SARS-CoV-2 using a competitive phage biopanning strategy
    Author
    Zeng, X;Li, L;Lin, J;Li, X;Liu, B;Kong, Y;Zeng, S;Du, J;Xiao, H;Zhang, T;Zhang, S;Liu, J;
    Year
    2020
    Journal
    Antibody Therapeutics
  • Development of novel detection system for sweet potato leaf curl virus using recombinant scFv
    Author
    Cho, SH;Kil, EJ;Cho, S;Byun, HS;Kang, EH;Choi, HS;Lee, MG;Lee, JS;Lee, YG;Lee, S;
    Year
    2020
    Journal
    Sci Rep
    Application
    phag Elisa
  • Abstracts from the 26th European Society for Animal Cell Technology Meeting - Cell culture technologies: bridging academia and industry to provide solutions for patients
    Author
    Pybus, LP;Hawke, E;Knowles, C;Kalsi, D;Barber, N;
    Year
    2020
    Journal
    BMC Proc
    Application
    cell panning

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Mouse MAb

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