Recombinant Anti-Interferon Gamma Antibody (FITC), Rabbit Monoclonal

1/1
Price:
Size:
Number:

Recombinant Anti-Interferon Gamma Antibody (FITC), Rabbit Monoclonal General Information

Product name
Recombinant Anti-Interferon Gamma Antibody (FITC), Rabbit Monoclonal
Validated applications
FCM
Species reactivity
Reacts with: Mouse
Specificity
Mouse Interferon Gamma
Immunogen
Recombinant Mouse IFNG / Interferon Gamma Protein (Catalog#50709-MNAH)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IFNG / Interferon Gamma (rM IFNG / Interferon Gamma; Catalog#50709-MNAH; NP_032363.1; Met1-Cys155) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Rabbit IgG Clone #348
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.03%ProClin300
Conjugate
FITC
Concentration
5 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze !

Recombinant Anti-Interferon Gamma Antibody (FITC), Rabbit Monoclonal Images

Flow cytometric analysis of Mouse Interferon gamma expression on BABL/c splenocytes. BABL/c splenocytes were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with FITC-conjugated anti-Mouse Interferon gamma and APC-conjugated anti-Human CD4. The dot plots were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-Interferon Gamma Antibody (FITC), Rabbit Monoclonal: Synonyms

Anti-Ifg Antibody; Anti-IFN-g Antibody

Interferon Gamma Background Information

IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.
Full Name
interferon, gamma
Research Areas
Related Pathways
  • IL12 signaling pathway
    IL12 signaling pathway
References
  • Gray P W, et al. (1982) Structure of the human immune interferon gene. Nature. 298: 859-63.
  • Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8.
  • Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7.
  • Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36.
  • Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7.
  • Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.