TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody

RRID Number: AB_2860178
EliteRmab®, manufactured by recombinant technology
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TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody General Information

Product name
TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Human
Specificity
Human TNFR2/TNFRSF1B
No cross-reactivity in ELISA with
Mouse TNFR2 (Catalog # 50128-M08H)
Immunogen
Recombinant Human TNFR2 protein (Catalog#10417-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human TNFRSF1B / CD120b (rh TNFRSF1B; Catalog#10417-H08H; Met 1-Asp 257; NP_001057.1).
Source
Monoclonal Rabbit IgG Clone #R00N6
Purification
Protein A
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
< 3 EU/mg
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody Validated Applications

Notes
Block: In a functional ELISA which immobilized recombinant Human TNFR2 (Catalog# 10417-H08H) at 1 μg/mL (100 µL/well) in the plate, the Rabbit anti-Human TNFR2 Monoclonal Antibody (Catalog# 10417-R00N6) can block the binding of 2 μg/mL of biotinylated Human TNFα (Catalog# 10602-HNAE-B) to Human TNFR2 , the EC50 is 2.07 μg/mL .
Neutralization: The neutralization activity of TNFR2/TNFRSF1B Neutralizing Antibody is Measured by its ability to neutralize TNFR2/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 1-3 µg/mL in the presence of 1 μg/mL Recombinant Human TNFR2/TNFRSF1B, 0.25 ng/mL Recombinant Human TNFa, and 1 µg/mL actinomycin D.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody Images

TNFR2/TNFRSF1B-mediated inhibition of cytotoxicity was Neutralized by Human TNFR2 Antibody. Recombinant Human TNFR2/TNFRSF1B (Catalog # 0417-H08H) inhibits Recombinant Human TNFa (Catalog # 10602-HNAE) induced cytotoxicity in the L-929 mouse fibroblast cell line. Inhibition of Recombinant Human TNFa (0.25 ng/mL) activity elicited by Recombinant Human TNFR2/TNFRSF1B (1 µg/mL) is neutralized by increasing concentrations of Human TNFR2/TNFRSF1B Monoclonal Antibody (Catalog # 0417-R00N6). The IC50 is typically 1-3 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody: Synonyms

Anti-CD120b Antibody; Anti-p75 Antibody; Anti-p75TNFR Antibody; Anti-TBPII Antibody; Anti-TNF-R-II Antibody; Anti-TNF-R75 Antibody; Anti-TNFBR Antibody; Anti-TNFR1B Antibody; Anti-TNFR2 Antibody; Anti-TNFR80 Antibody

TNFR2/TNFRSF1B Background Information

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Full Name
tumor necrosis factor receptor superfamily, member 1b
Research Areas
References
  • Komata T, et al. (1999) Association of tumor necrosis factor receptor 2 (TNFR2) polymorphism with susceptibility to systemic lupus erythematosus. Tissue Antigens. 53(6): 527-33.
  • Tsuchiya N, et al. (2001) Analysis of the association of HLA-DRB1, TNFalpha promoter and TNFR2 (TNFRSF1B) polymorphisms with SLE using transmission disequilibrium test. Genes Immun. 2(6): 317-22.
  • Guo G, et al. (1999) Role of TNFR1 and TNFR2 receptors in tubulointerstitial fibrosis of obstructive nephropathy. Am J Physiol. 277(5): 766-72.
  • The TNF/TNFR2 signaling pathway is a key regulatory factor in endothelial progenitor cell immunosuppressive effect
    Author
    Naserian, S;Abdelgawad, ME;Afshar Bakshloo, M;Ha, G;Arouche, N;Cohen, JL;Salomon, BL;Uzan, G;
    Year
    2020
    Journal
    Cell Commun. Signal
    Application
    co-culture
  • TNFα priming through its interaction with TNFR2 enhances Endothelial Progenitor Cell immunosuppressive effect: a new hope for their widespread clinical application
    Author
    BARKESTANI, MN;SHAMDANI, S;BAKSHLOO, MA;
    Year
    2020
    Journal
    Research Square
    Application
    blocking

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