Lane A: Mouse heart Tissue lysateLysates/proteins at 30 μg per lane.Secondary
Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:32 kDa
Observed band size:30 kDa
(We are unsure as to the identity of these extra bands.)