Anti-Insulin Receptor Antibody (PE)

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Anti-Insulin Receptor Antibody (PE) (Mouse Monoclonal antibody) General Information

Product name
Anti-Insulin Receptor Antibody (PE)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human Insulin Receptor
Immunogen
Recombinant Human Insulin Receptor/INSR/CD220 Protein (Catalog#11086-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Insulin Receptor/INSR/CD220 (rh Insulin Receptor/INSR/CD220; Catalog#11086-H08H; NP_001073285.1; Met1-Lys944) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Source
Monoclonal Mouse IgG1 Clone #01
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
PE
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-Insulin Receptor Antibody (PE) (Mouse Monoclonal antibody) Images

Flow cytometric analysis of Human INSR(CD220) expression on human whole blood monocytes. Cells were stained with PE-conjugated anti-Human INSR(CD220). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.

Anti-Insulin Receptor Antibody (PE): Synonyms

Anti-CD220 Antibody; Anti-HHF5 Antibody; Anti-Insulin Receptor Antibody

Insulin Receptor Background Information

INSR (Insulin receptor), also known as CD22, is a transmembrane receptor that is activated by insulin. INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K). IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5). It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM).
Full Name
insulin receptor
Related Pathways
  • AMPK Signaling Pathway
    AMPK Signaling Pathway
  • Autophagy Pathway
    Autophagy Pathway
References
  • Ebina Y., et al.,(1985), The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40:747-758.
  • Ullrich A., et al., (1985), Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.Nature 313:756-761.
  • Grimwood J., et al.,(2004), The DNA sequence and biology of human chromosome 19.Nature 428:529-535.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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