Anti-CTLA-4 Antibody

1/1
Price:
Size:
Number:

Anti-CTLA-4 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-CTLA-4 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human CTLA-4
Immunogen
Recombinant Human CTLA-4 / CD152 Protein (Catalog#11159-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CTLA-4 / CD152 (rh CTLA-4 / CD152; Catalog#11159-H08H; NP_005205.2; Met1-Phe162). CTLA-4 / CD152 specific IgG was purified by Human CTLA-4 / CD152 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CTLA-4 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CTLA-4 Antibody (Rabbit Polyclonal antibody) Images

Anti-CTLA4 rabbit polyclonal antibody at 1:500 dilution.

Lane A: MOLT4 Membrane Lysate

Lysates/proteins at 30 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:25 kDa

Observed band size:23 kDa

Anti-CTLA-4 Antibody: Synonyms

Anti-ALPS5 Antibody; Anti-CD Antibody; Anti-CD152 Antibody; Anti-CELIAC3 Antibody; Anti-CTLA-4 Antibody; Anti-GRD4 Antibody; Anti-GSE Antibody; Anti-IDDM12 Antibody

CTLA-4 Background Information

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD8 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).
Full Name
cytotoxic T-lymphocyte-associated protein 4
References
  • Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24.
  • Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33.
  • Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.