Anti-CD73 Antibody (FITC)

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Anti-CD73 Antibody (FITC) (Mouse Monoclonal antibody) General Information

Product name
Anti-CD73 Antibody (FITC)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human CD73
Immunogen
Recombinant Human CD73 / NT5E Protein (Catalog#10904-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; Catalog#10904-H08H; NP_002517.1; Met1-Lys547) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Mouse IgG1 Clone #07
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.03%ProClin300
Conjugate
FITC
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze !

Anti-CD73 Antibody (FITC) (Mouse Monoclonal antibody) Images

Flow cytometric analysis of Human NT5E(CD73) expression on human whole blood lymphocytes. Cells were stained with FITC-conjugated anti-Human NT5E(CD73). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

Anti-CD73 Antibody (FITC): Synonyms

Anti-CALJA Antibody; Anti-CD73 Antibody; Anti-E5NT Antibody; Anti-eN Antibody; Anti-eNT Antibody; Anti-NT Antibody; Anti-NT5 Antibody; Anti-NTE Antibody

CD73 Background Information

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
Full Name
5'-nucleotidase, ecto (CD73)
References
  • Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
  • Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
  • Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
  • Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.

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