Anti-CD45 Antibody (FITC), Mouse Monoclonal

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Anti-CD45 Antibody (FITC), Mouse Monoclonal General Information

Product name
Anti-CD45 Antibody (FITC), Mouse Monoclonal
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human CD45
Immunogen
Recombinant Human CD45 protein (Catalog#10086-H02H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD45 (rh CD45; Catalog#10086-H02H; NP_563578.1; Met1-Lys416) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Mouse IgG1 Clone #05
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
FITC
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-CD45 Antibody (FITC), Mouse Monoclonal Images

Flow cytometric analysis of Human CD45 expression on human whole blood lymphocytes. Cells were stained with FITC-conjugated anti-Human CD45. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

Anti-CD45 Antibody (FITC), Mouse Monoclonal: Synonyms

Anti-B220 Antibody; Anti-CD45 Antibody; Anti-CD45R Antibody; Anti-GP180 Antibody; Anti-L-CA Antibody; Anti-LCA Antibody; Anti-LY5 Antibody; Anti-T200 Antibody

CD45 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.
Full Name
protein tyrosine phosphatase, receptor type, C
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Irie-Sasaki J, et al. (2001) CD45 is a JAK phosphatase and negatively regulates cytokine receptor signaling. Nature. 409: 349-54.
  • Peptide-based isolation of circulating tumor cells by magnetic nanoparticles
    Author
    Bai, L;Du, Y;Peng, J;Liu, Y;Wang, Y;Yang, Y;
    Year
    2014
    Journal
    Journal of Materials Chemistry B
    Application
    FCM
  • Generation of a monoclonal antibody recognizing the heavily glycosylated CD45 protein and its application on identifying circulating tumor cells
    Author
    Zhang, W;Li, Z;Wang, Z;Yue, C;Zheng, H;Li, R;Zhou, M;Hu, Z;Wei, Z;Li, Q;
    Year
    2018
    Journal
    PLoS ONE
    Application
    FCM

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