Anti-CD45 Antibody, Mouse Monoclonal
RRID Number: AB_2860081
Cat: 10086-MM05
Anti-CD45 Antibody, Mouse Monoclonal General Information
Product name
Anti-CD45 Antibody, Mouse Monoclonal
Validated applications
ELISA,FCM,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human CD45
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
Immunogen
Recombinant Human CD45 protein (Catalog#10086-H02H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD45 (rh CD45; Catalog#10086-H02H; NP_563578.1; Met1-Lys416). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-CD45 Antibody, Mouse Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| ELISA | 1:1000-1:2000 |
| ICC-IF | 1:20-1:100 |
| FCM | 1:25-1:100 |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-CD45 Antibody, Mouse Monoclonal Images
Flow cytometric analysis of Human CD45 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD45, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of Human CD45 in jurkat cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with mouse anti-Human CD45 monoclonal antibody (dilution ratio 1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to membrane.
Anti-CD45 Antibody, Mouse Monoclonal: Synonyms
Anti-B220 Antibody; Anti-CD45 Antibody; Anti-CD45R Antibody; Anti-GP180 Antibody; Anti-L-CA Antibody; Anti-LCA Antibody; Anti-LY5 Antibody; Anti-T200 Antibody
CD45 Background Information
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.
Full Name
protein tyrosine phosphatase, receptor type, C
Research Areas
Related Pathways
-
B Cell Receptor Signaling Pathway -
Jak-Stat Signaling Pathway -
T Cell Receptor Signaling Pathway -
Common G-Chain signaling pathway
References
- Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
- Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
- Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
- Irie-Sasaki J, et al. (2001) CD45 is a JAK phosphatase and negatively regulates cytokine receptor signaling. Nature. 409: 349-54.
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