Anti-CD45 Antibody, Mouse Monoclonal

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Anti-CD45 Antibody, Mouse Monoclonal General Information

Product name
Anti-CD45 Antibody, Mouse Monoclonal
Validated applications
IHC-P
Species reactivity
Reacts with: Human
Specificity
Human CD45
Immunogen
A synthetic peptide corresponding to the center region of the Human CD45 / PTPRC
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the Human CD45 / PTPRC. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #18
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD45 Antibody, Mouse Monoclonal Validated Applications

Application Dilution
IHC-P 1:50-1:200
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD45 Antibody, Mouse Monoclonal Images

Immunochemical staining of human LCA in human spleen with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).
Immunochemical staining of human LCA in human tonsil with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).

Anti-CD45 Antibody, Mouse Monoclonal: Synonyms

Anti-B220 Antibody; Anti-CD45 Antibody; Anti-CD45R Antibody; Anti-GP180 Antibody; Anti-L-CA Antibody; Anti-LCA Antibody; Anti-LY5 Antibody; Anti-T200 Antibody

CD45 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.
Full Name
protein tyrosine phosphatase, receptor type, C
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Irie-Sasaki J, et al. (2001) CD45 is a JAK phosphatase and negatively regulates cytokine receptor signaling. Nature. 409: 349-54.

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Mouse MAb

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