Human CD3 (humanized OKT3) Antibody-Human IgG4 (GMP-grade) General Information
Human CD3 (humanized OKT3) Antibody-Human IgG4 (GMP-grade)
Reacts with: Human
Human CD3 epsilon/CD3e
Human CD3 (humanized OKT3) Antibody is a humanized antibody of Orthoclone OKT3 (GMP-grade procution). Orthoclone OKT3 is an immunosuppressant drug given to reduce acute rejection in patients with organ transplants. It is a monoclonal antibody targeted at the CD3 receptor, a membrane protein on the surface of T cells. It was the first monoclonal antibody to be approved for clinical use in humans.
Human IgG4 Clone #H001
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
< 0.5 EU/mg
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Human CD3 (humanized OKT3) Antibody-Human IgG4 (GMP-grade) Validated Applications
Agonist Activity (Func/A)：
Agonist Activity – Measured by its ability to induce proliferation of human T cells. The ED50 for this effect is typically 2-10 ug/mL.
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Human CD3 (humanized OKT3) Antibody-Human IgG4 (GMP-grade) Images
The staining effect of Human CD3 (humanized OKT3) antibody to CD3 of Human Peripheral Blood Lymphocytes by Flow cytometric analysis. A: Lymphocytes were stained with PE-conjugated human CD3 (humanized OKT3) antibody. B: The binding effect of PE-conjugated humanized OKT3 antibody(Red histograms) in the lymphocytes can be blocked by pre-incubation of the CD3 antibody (Miltenyi Biotec, 130-093-387) for 20min (blue histograms).
CD3+ T cell staining of lymphocytes using Human CD3 (humanized OKT3) antibody by Flow cytometric analysis. A: Lymphocytes were stained with both PE-conjugated CD3(humanized OKT3) antibody and OKT3-FITC-eBioscience (eBioscience, 11-0037-41). B: Lymphocytes were stained with PE-conjugated humanized OKT3 antibody and CD19-APC (BD, 555415).
Effect of Human CD3 (humanized OKT3) antibody on the proliferation and IFN-gamma secretion of CD4+ T cell. The isolated CD4+ T cells(1e5/well) were cultured for 4 days in the 96-well cell plates coated with serial dilutions of CD3 (humanized OKT3) antibody. A. The cell proliferation level was detected using MTT detection; B. IFN-gamma secretion in the medium was measured by IFN-gamma Quantitative ELISA Kit.
Using the Octet RED System, the affinity constant (Kd) of CD3e(Cat: 10977-H08H) bound to Human CD3 (humanized OKT3) Antibody-Human IgG4 (Cat: GMP-10977-H001) was 3.8nM.
T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.
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